NFKB1 and cervical carcinoma: To investigate the role of NF-κB in cervical cancer cells, we constructed an NF-κB overexpression vector (pcDNA3/NF-κB) and an NF-κB interference vector (pSilencer/shR-NF-κB) which over express or knock down p50 protein that were validated by western blot in transfected HeLa and C33A cells (Figure 1A and B).