To investigate the role of NF-κB in cervical cancer cells, we constructed an NF-κB overexpression vector (pcDNA3/NF-κB) and an NF-κB interference vector (pSilencer/shR-NF-κB) which over express or knock down p50 protein that were validated by western blot in transfected HeLa and C33A cells (Figure 1A and B). This evidence concerns the gene NFKB1 and cervical cancer.