To investigate whether loss of other genes besides LSAMP could be important, we performed gene expression analysis of LSAMP RNA antisense 1 (LSAMP-AS1), LSAMP-AS3 and LSAMP-AS4 in a panel of 5 osteosarcoma clinical samples, 13 xenograft samples, 19 cell lines and 14 control samples (n = 51). The gene discussed is LSAMP; the disease is osteosarcoma.