Effects of longer survival time in vivo could be observed as early as 4 weeks and last to 8 weeks which assured the stable and longer secretion of HGF by MSCs to improve the bone marrow microenvironment and promote proliferation and differentiation of themselves as well as other cells in the femoral head, thereby promoting amelioration of ANFH. This evidence concerns the gene HGF and familial avascular necrosis of femoral head.