It should be noted that BACE1 levels were significantly increased in the brains of D257A; APP/Ld and APP/Ld compared to D257A and wild-type mice, consistent with our previous work showing that BACE1 levels are elevated by amyloid pathology in human AD and APP transgenic mouse brains[32,33,53]. This evidence concerns the gene BACE1 and amyloidosis.