CD40 and metastatic malignant neoplasm in the lymph nodes: The positive rate of CD40 mutant expression: (1) in cases with tumor invasion to deep muscle layer was significantly higher than that in cases with tumor invasion not to deep muscle layer (61.8% vs. 10%, χ2 = 9.444, P = 0.002); (2) in cases with lymph node metastasis was significantly higher than that in cases without lymph node metastasis (65% vs. 37%, χ2 = 7.802, P = 0.005); (3) in cases of stage III and IV was significantly higher than that in cases of stages I and II (63.9% vs. 23.5%, χ2 = 8.774, P = 0.003).