TP53 and lung carcinoma: Similarly, the human lung cancer cell line H1299 (TP53 null) transfected with p53 and treated with nutlin-3, followed by immunoprecipitation with an anti-acetyl-lysine antibody, demonstrated a high increase in levels of acetylated p53 after nutlin-treatment, and only a small increase in total levels of p53 (Figure 1C).