Again, we observed that high density in 3D conditions evoked NED in both AR-positive and AR-negative cell lines, as in 2D conditions (Figure 1 and Additional file 1: Figure S1B-D), because cultivation in 3D at high density also increased the expression of NED markers at both protein and mRNA level in the AR-negative prostate cell line BPH-1 and its tumorigenic clone CAFTD03, as well as in the prostate cancer cell lines PC3 and DU-145 (Figure 2C). Here, AR is linked to prostate carcinoma.