We utilized several reproducible and sensitive assays, both biochemical and cell biological, to investigate the effects of non-synonymous GCKR variants first identified in individuals with hypertriglyceridemia, including novel assays to measure variants simultaneously in a high-throughput manner (Fig. 2; Supplementary Material, Fig. S1), which provided a highly sensitive platform to compare the effects of different variants. The gene discussed is GCKR; the disease is hypertriglyceridemia.