Our results confirmed the activation of the Smad3/TGFβ pathway, showing a strong increase in 12xSBE:mCherry expression rising at 16 wpf both in tumor mass (eGFP-KRASG12D-positive cells) and stromal microenvironment (eGFP-KRASG12D-negative cells) (supplementary material Fig. S6A,B; Movie 2). This evidence concerns the gene SMAD3 and neoplasm.