In this work, a new zebrafish model of pancreatic adenocarcinoma was reproduced by using the conditional Gal4/UAS expression system (Liu and Leach, 2011), driving the expression of mutated KRASG12D in the exocrine pancreas of zebrafish derived from outcrosses with TGFβ, Notch, Bmp and Shh reporter lines expressing the fluorescent reporter mCherry. Here, SHH is linked to pancreatic adenocarcinoma.