In order to identify which of these pathways are involved in pancreatic cancer onset, we performed confocal microscope analysis at 3, 5, 7, 30 and 60 days post fertilization (dpf), by using 10 eGFP-positive larvae from Tg(ptf1a:eGFP) and Tg(ptf1a:Gal4)/UAS:eGFP-KRASG12D injected lines for each time point. This evidence concerns the gene PTF1A and familial pancreatic carcinoma.