By surface engineering of uniform HMSN with radioisotopes (i.e.64Cu), NIR dyes (i.e. ZW800) and CD105 targeting antibodies (i.e. TRC105), we reported here the first example of in vivo tumor vasculature targeted PET/NIRF dual-modal imaging of 64Cu-HMSN-ZW800-TRC105. This evidence concerns the gene ENG and neoplasm.