Figure 3A illustrates this using western blot analysis of rpS7 expression in wild type or rpS7-deficient embryos at 1 and 2 days post fertilization (dpf). When normalized to actin, rpS7 expression at 1 dpf in the rpS7-deficient embryos is ∼57% ±5 (N = 3) of the expression level in wild type embryos. By 2 dpf, this drops to less than 5%. We therefore examined the red blood cells (RBCs) at 1 dpf, when rpS7 levels in the mutant embryos are characteristic of RPS7 haploinsufficiency seen in DBA patients [3]. The gene discussed is RPS7; the disease is Diamond-Blackfan anemia.