These findings were corroborated upon ectopic expression of MYC-SIRT1 and FLAG-FOXA2 in human osteosarcoma U2OS cells (which do not express FOXA2 endogenously), as we found a specific interaction of FLAG-FOXA2 and MYC-SIRT1 by using FLAG- and SIRT1-specific antibodies (data not shown). This evidence concerns the gene FOXA2 and osteosarcoma.