While accumulated nuclear localization of IE62 was observed for melanoma cells transfected with pOka-TK-GFP BAC and all three mutants, only pOka-TK-GFP, pOka-TK-GFP-gH[Δ834-841], and pOka-TK-GFP-gB[Y881F] had cytoplasmic localization of IE62, suggesting that the gB[Y881F]/gH[Δ834-841] mutations prevented the cell from entering the late stages of VZV infection. The gene discussed is TKT; the disease is melanoma.