Several amplified genes were linked to metabolic pathways, including the NADH dehydrogenase subunit NDUFC2 and the AMP-activated protein kinase subunit PRKAB2. Cancer cells utilize distinct energy production mechanisms compared to normal cells, such as the high rate of aerobic glycolysis (termed the Warburg effect), and these mechanisms can be utilized to diagnose and treat cancers [30]. The gene discussed is PRKAB2; the disease is cancer.