In this sense, OPN-deficiency prevented the HFD-induced increase in extracellular matrix proteins such as Col1a1, Col6a3 and Eln. Moreover Col6a1 and markers of liver fibrosis such as α-SMA and annexin 2 [50] decreased by OPN-deletion regardless of diet. The gene discussed is COL6A3; the disease is Hepatic fibrosis.