Each construct contained a portion of the intracellular (Lm-LLO-IC1 and Lm-LLO-IC2) or the extracellular (Lm-LLO-EC1, Lm-LLO-EC2 and Lm-LLO-EC3) portion of the full-length rat Her2/neu protein to allow for efficient production and secretion by Lm. Therapeutic vaccination with each of the constructs individually resulted in reduced or static growth of NT-2 breast tumors, a tumor cell that constitutively expresses rat Her2/neu under the mouse mammary tumor virus (MMTV) promoter (Singh et al., 2005). Here, ERBB2 is linked to breast neoplasm.