However, we did not find the sensitivity difference between the sequential calretinin and CK5/6 criteria and the others, including positive calretinin stain alone (52.17%), CK5/6 stained negative or 1+ (60.0%) and less CK5/6 stain in CC than NBD (56.67%) (Table 4). The gene discussed is KRT5; the disease is cholangiocarcinoma.