ESR1 and lobular breast carcinoma in situ: Pre-malignant BCs such as atypical ductal hyperplasia, ductal carcinoma in situ (DCIS) and LCIS highly express ER, and none, low or modest ER-expressing cells are detected in invasive breast carcinoma (IBC) [34], suggesting a possibility that ER-positive BC seems to be at early stage of cancer.