CKAP2 and adult-onset autosomal dominant demyelinating leukodystrophy: Consistent with the EM data (Fig. 1D, E), the sequential biochemical extraction of nuclear proteins with increasing salt concentration revealed that the majority of LB1 was in the insoluble fraction of both CTR and ADLD fibroblasts, indicating that LB1 primarily associates with the nuclear lamina (Supplemental Fig. S3A).