As shown in Figures 4, we found that HF feeding (Figure 4B, p<0.004) and obesity (Figure 4C, p<0.03) were associated with a 60% decrease in the relative levels of ACC in mammary tissue extracts compared to LF-fed and HF-Ln mammary glands, respectively. The gene discussed is ACACA; the disease is obesity due to melanocortin 4 receptor deficiency.