Figure 1A showed that HIPK2 expression in patients who are chemo-resistant is significantly decreased compared with chemo-sensitive patients. Then we established a cisplatin-resistant subline from the human bladder cancer cell line RT4 (RT4-CisR), and assayed the expression level of HIPK2. As shown in Figure 1B, HIPK2 mRNA levels were lower in RT4-CisR cells compared with RT4 cells. Similarly, HIPK2 protein levels were downregulated in RT4-CisR cells (Figure 1C). This evidence concerns the gene HIPK2 and urinary bladder cancer.