On the other hand, Interferon-γ (IFN-γ) is an important cytokine which can enhance the antitumor effects of antimetabolite on cancer cells and induce or modulate apoptosis either as a single agent or in combination with tumor necrosis factor-α (TNF-α), which is another kind of cytokine produced by activated monocytes and phagocytes that could effectively kill many sorts of cancer cells both in vivo and in vitro32, 33, 34, 35. This evidence concerns the gene TNF and cancer.