FUS and amyotrophic lateral sclerosis: To test if FGs display the same features, we isolated granule-enriched fraction from RNase A-treated or untreated lysates of cells expressing cytoplasmic forms of the protein, either FUS R522G or FUS lacking a portion of RGG3 (dRGG, corresponding to a familial ALS-associated mutant variant G466VfsX14).