Consistent with the results of previous studies (15,16,19,25), GFP-tagged FUS variants rendered cytoplasmic by the introduction of mutations or truncations abrogating nuclear import were diffusely distributed in the cytoplasm of SH-SY5Y neuroblastoma cells or primary hippocampal neurons (Fig. 1A, Supplementary Material, Fig. S1A). Here, FUS is linked to neuroblastoma.