MYC and osteosarcoma: On the contrary, the mRNA level of the Wnt target gene c-MYC was unaffected by overnight compound treatment in U2OS cells (Figure 3A) as well as in all the osteosarcoma lines tested in the soft agar assay (data not shown), arguing that it might not represent a direct transcriptional target of the Wnt signaling in the analyzed tumor cells.