He et al. have reported the serum biomarker screening of AFP negative HBV related HCC through the iTRAQ based approach [35]; Ko et al. have reported the iTRAQ based quantitative analysis of HCC cancer stem cell proteome [36]; Huang et al. have reported the iTRAQ based serum biomarker screening of the HCC micro-vascular invasion [37]; Qin et al., Yu et al. and Wang et al. have reported the screening of metastatic related proteins of HCC through iTRAQ based approach [33,38,39]; the iTRAQ based quantitative study of proteome change during HBV infection has also been reported [40,41]. This evidence concerns the gene AFP and hepatocellular carcinoma.