In our study, we identified that protein S100A12 and AMACR were reversely expressed in the early (within 6 months, down regulated) and late (from 6 to 12 months, up regulated) recurrence/metastasis H-HCC patients; and further validated these findings from clinical samples at the level of both mRNA and protein expression. The gene discussed is AMACR; the disease is hepatocellular carcinoma.