Therefore, we speculate that upregulation of SATB1 may contribute to the initialization of EMT process during the invasion and metastasis of RCC, which may explain our observation that SATB1 expression was dramatically associated with invasion and lymph node metastasis of ccRCC and that SATB1 expression was positively correlated with ZEB2 expression and inversely correlated with the level of E-cadherin in our ccRCC cohort, respectively. This evidence concerns the gene ZEB2 and renal cell carcinoma.