To investigate the phenomenon and mechanism of SATB1-mediated regulation on invasive and metastatic capacity of ccRCC, additional immunohistochemical staining were utilized to detect the levels of EMT markers (including E-cadherin, ZEB2, vimentin and fibronectin) and SATB2, a homologue of SATB1, and analyze the potential correlations between SATB1 and expressions of EMT markers or SATB2 in ccRCC tissues. This evidence concerns the gene CDH1 and nonpapillary renal cell carcinoma.