Furthermore, SATB1 expression was detected in established human RCC cell lines including 786-O, A498 and ACHN to investigate the roles of SATB1 expression in biologic behavior of renal cancer cells by a combination of western blotting, quantitative real-time PCR, immunofluorescence staining, cell proliferation, migration and invasion assays. This evidence concerns the gene SATB1 and renal cell carcinoma.