We detected the qualitative degrees of RNF180 promoter methylation in 67 gastric cancer tissues with the MSP analysis (including 12 cases with methylation, 33 cases with partial methylation, and 22 cases without non-methylation), while no RNF180 promoter methylation was found in 25 normal gastric mucosal tissues (Figure 3). The gene discussed is RNF180; the disease is gastric cancer.