JARID2 and infection: Parallel experiments provided additional support to this conclusion by showing that both the knockdown of JARID2 and the overexpression of the histone H3K27me3 demethylase JMJD3 also inhibit the activation of the HCMV MIEP (Fig. 2B and Fig.S2B).To determine whether blocking histone H3K27 trimethylation inhibits or simply delays the expression of IE1, we knocked down EZH2, or NDY1/KDM2B, in HFFs and we examined the expression of IE1 at multiple time points from the start of the infection.