The finding that LieIF/IFN-γ- pre-treated J774A.1 cells showed resistance to L. donovani infection prompted us to examine the efficacy of LieIF on intracellular growth of L. donovani following infection, and we showed that J774A.1 cells treatment with LieIF/IFN-γ after Leishmania infection also led to a significant prevention of parasite growth at early (19 h) and late time (72 h) points of infection. This evidence concerns the gene IFNG and infection.