To determine the effect of introducing cleaved GroEL2 into the hip1 mutant, we infected macrophages derived from the bone marrow of C57BL/6 mice with wild type, hip1 mutant, hip1 mutant complemented with Hip1, or hip1 mutant complemented with cleaved GroEL2, and assayed for the production of the proinflammatory cytokines IL-6, IL-1β, and TNF-α in macrophage supernatants, 24 hours post-infection. The gene discussed is TNF; the disease is infection.