PLVAP and cytomegalovirus infection: To extend the data to HCMV infection and to test BW:FcγRIIA-ζ cells, a polyclonal antibody preparation (IVIG, Cytotect) was used to opsonize MRC-5 fibroblasts infected with the HCMV HB5ΔIRLΔgp68/Δgp34 mutant lacking both gp68 and gp34.