RASSF1A was methylated in 14/27 (52%) of HCC samples and in 1/17 (6%) of adjacent normal samples. B4GALT1 was methylated in 14/27 (52%) of HCC samples and in 0/20 (0%) of adjacent normal samples. SSBP2 was methylated in 14/27 (52%) of HCC samples and in 6/18 (33%) of adjacent normal samples. This evidence concerns the gene SSBP2 and hepatocellular carcinoma.