The log10 values of parasite load in footpads correlated significantly with the increase in IDR (R = −0.6734; p < 0.0001; R2 = 0.4534) and with the decrease in footpad lesions (R = 0.5994; p < 0.0001; R2 = 0.3593) confirming that NH36 vaccine generated cross-protection against cutaneous leishmaniasis is determined by CD8 epitopes of F1 domain and by CD4 epitopes in the F3 domain. The gene discussed is CD4; the disease is cutaneous leishmaniasis.