In our previous report, we found that three cancer cell lines (i.e., epidermal carcinoma A431 cells, esophageal carcinoma EC109 cells, and thyroid carcinoma TT cells) acquired cell motility by EGF stimulation, but chemosensitivity cluster analysis showed that A431 cells and EC109 cells are clustered into the same cluster, on the other hand, TT cells are classified into the different cluster. The gene discussed is EGF; the disease is cancer.