Figure 2 shows the 2 h post-treatment time-point for both LNCaP and DU145 cells treated with muJ591:MIRB complex. AlexaFluor-488 staining allowed for localization of the antibody within sub-cellular and extracellular regions of PSMA-expressing LNCaP cells (Figure 2A). The Rhodamine-B fluorophore (red) that is attached to MIRB was specifically detected in PSMA-expressing LNCaP cells (Figure 2C). No red or green fluorescent signals were observed in DU145 control cell line (Figure 2B and 2D) suggesting high specificity of muJ591:MIRB complex in detecting PSMA-positive prostate cancer cells. The gene discussed is FOLH1; the disease is Familial prostate cancer.