The percentage of PCNA-positive cells of colon mucosa in normal control group were 3.4±0.1%, whereas the percentage of PCNA-positive cells in AOM control group were 48.5±2.5%, 5-fluorouracil 11.67±1.03%, low and high-doses of A. ilicifolius- treated groups were 28.2±2.75% and 33.6±2.82%, respectively. This evidence concerns the gene PCNA and infectious otitis media.