To evaluate the effect of B-cell depletion therapy with RTX on autoimmune serological memory, we assessed IgM-RF and IgG-anti-CCP levels (relevant auto-Abs in RA) and IgG-anti-dsDNA levels (relevant auto-Ab in SLE) in patients with the corresponding diagnoses before and after RTX treatment, and in HV as controls. This evidence concerns the gene CD40LG and systemic lupus erythematosus.