The TUNEL staining assay indicated that the blockages of P2X7 and especially caspase-8 could significantly inhibit the apoptotic process induced by the cryoablated tumor extracts in GL261 cells, while caspase-9 blockage did not affect the apoptosis-triggering effects of these extracts (Figure 2(e)). Here, P2RX7 is linked to neoplasm.