Overexpression of wildtype NLK in the breast cancer cell lines MCF7, or non-cancerous human breast epithelial cells MCF10A, displayed a dominant cytosolic accumulation in all cells (Figure 2A), whereas in the NLK mutants, the distribution was mainly located to the nuclei of MCF7 and MCF10A cells (Figure 2A). This evidence concerns the gene NLK and breast cancer.