In ICC, including E-cadherin-positive and E-cadherin-negative expression cases, β-catenin was strongly expressed at the cell membrane, and nuclear accumulation of this protein was not elevated with E-cadherin loss, suggesting that E-cadherin loss was not sufficient per se for nuclear translocation of unbound β-catenin. Here, CDH1 is linked to intrahepatic cholangiocarcinoma.