The results in this study showed that highly aggressive GBC-SD cells formed in vivo VM networks by overexpressing EphA2, FAK and Paxillin-P proteins/mRNAs; NCTD downregulated expression of these VM signaling-related markers in vivo, thus similarly to TIMP-2, inhibited VM formation of GBC-SD cells and growth of GBC-SD xenografts. This evidence concerns the gene PTK2 and Salla disease.