PTK2 and neoplasm: To investigate the underlying mechanisms of NCTD effects on tumor growth and VM of human gallbladder cancers, in this study we explored the in vivo regulation effect of NCTD on the EphA2/FAK/Paxillin signaling pathway, i.e., expression of VM signaling-related markers EphA2, FAK and Paxillin-P at protein and mRNA levels of GBC-SD xenografts by indirect immunofluorescence method, western blotting and RT-PCR.