The results shown that highly aggressive GBC-SD cells were able to form VM network structures when injected subcutaneously into the mice, and facilitated growth of GBC-SD xenografts; that NCTD, similar to TIMP-2, inhibited the VM formation of GBC-SD xenografts, and then suppressed tumor growth of the xenografts effectively and safely in vivo. Here, TIMP2 is linked to neoplasm.