To further simplify the use of the UCP-LFA for leprosy diagnostics in a field setting, we next developed a multiplex UCP-LFA for simultaneous detection of anti-PGL-I antibodies and IP-10 in whole blood samples, analogous to the earlier described anti-PGL-I/IL-10 multiplex UCP-LFA [26]. The gene discussed is CXCL10; the disease is leprosy.