Regulation of apoptosis in pancreatic cancer cells was analysed upon IGF-1R silencing using Annexin V-FITC Apoptosis Detection Kit I. Flow cytometric analysis showed a pronounced induction of apoptosis/cell death by IGF-1R silencing in pancreatic cancer cells (45.4%, 55.4% in PANC-1 and 47.7%, 59.5% in HPAC was observed after 48 h and 72 h of post transfection, respectively) (Figure 4A & B). This evidence concerns the gene IGF1R and familial pancreatic carcinoma.