In this study, in an HPV16-positive experimental murine tumor model established with the BMK-16/myc cell line, the application of naked cDNA that encodes IL-12 induced inhibition of tumor growth and favored the expression of IL-2, IL-12, and IFN-γ and the low-level expression of IL-10, IL-4, and TGF-β1 cytokines. Here, TGFB1 is linked to neoplasm.