However, the expression levels of phosphorylated Akt and phosphorylated eNOS were significantly lower in the mice with MI compared with the sham-operated group (P<0.05); treatment with muscone increased the phosphorylation of Akt (1.968±0.2110 vs. 1.286±0.04034; MD 0.6825±0.2149; 95% CI 0.08610–1.279; P=0.0336) and phosphorylated eNOS (2.347±0.2729 vs. 1.537±0.1645; MD 0.8095±0.3186; 95% CI 0.02995–1.589; P=0.044) compared to the untreated group (Fig. 6). The gene discussed is NOS3; the disease is myocardial infarction.