Here we identified that GLA attenuated (a) the expressions of CD44, CD133, CD90, and EpCAM, (b) the capacity of spheroids formation (a marker for self-renewal), and (c) the capacity of anchorage-independent growth (a character of malignant cells) in HepG2, Huh-7, and MHCC97H cells, suggesting a novel function that GLA could regulate the CSCs-like properties in HCC cells. Here, EPCAM is linked to hepatocellular carcinoma.