To confirm the regulation of EGFR by PI4KIIα, we tested this effect in different cell lines: MCF-7 cells (human breast adenocarcinoma cell line, Fig. 2A), MDA-MB-231 cells (triple-negative breast cancer cell line, Fig. S1A) and A549 cells (non-small-cell lung carcinoma (NSCLC) cell line, Fig. S1B), and all results confirmed that PI4KIIα knockdown resulted in EGFR reduction. The gene discussed is EGFR; the disease is triple-negative breast carcinoma.