Another study of gastric cardia adenocarcinoma demonstrated that while TSP1 promoter methylation affected the mRNA and protein levels of thrombospondin 1, there were no significant effects on TGFβ expression, although a nonsignificant decrease of active TGFβ was seen in patients with TSP1 hypermethylation [64] indicating that the methylation of TSP1 causes downregulation of TSP1 and therefore decreased active TGFβ. The gene discussed is TGFB1; the disease is gastric cardia adenocarcinoma.