The use of glaucomatous-like stimulus (cyclical mechanical strain—cell stretch and a hypoxic environment—1% O2) upregulated genes associated with the ECM, such as CTGF, Collagen I, Elastin, TSP-1 macrophage migration inhibitory factor 1 (MIF), discoidin domain receptor family member 1 (DDR1/TrkE), and Insulin-like growth factor 2 receptor (IGFR2) seen in the LC region of glaucoma [84, 100]. Here, CCN2 is linked to glaucoma.