Interestingly, using TALENs to known oncogenes of WNT/Beta-catenin signaling (MYC and CTNNB1), recently reported to be critical for MPNST cell maintenance, demonstrated near undetectable levels of gene modification in MPNST cells without co-transposition; though these TALENs were previously validated in U2OS osteosarcoma cells and produced robust gene modification by transient transfection (13% and 26% for MYC and CTNNB1, respectively)[26],[27]. The gene discussed is MYC; the disease is osteosarcoma.