To elucidate the intriguing function of upregulated Orai1 but not STIM1 in esophageal cancer, the Orai1-mediated SOCE activity were examined in epithelial cell lines derived from human ESCC tumors: KYSE-150, KYSE-190, KYSE-30, KYSE-510 and KYSE-790. This evidence concerns the gene STIM1 and esophageal cancer.